An FeV(O) complex has been synthesized from equimolar solutions of (Et4N)2[FeIII(Cl)(biuret-amide)] and mCPBA in CH3CN at room temperature. The FeV(O) complex has been characterized by UV-vis, EPR, M{\"{o}}ssbauer and HRMS and shown to be capable of oxidizing a series of alkanes having C-H bond dissociation energies ranging from 99.3 kcal mol-1 (cyclohexane) to 84.5 kcal mol-1 (cumene). Linearity in the Bell-Evans-Polayni graph and the finding of a large kinetic isotope effect suggest that hydrogen abstraction is engaged the rate-determining step. {\textcopyright} 2014 American Chemical Society.